Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
Identifieur interne : 003C28 ( Main/Repository ); précédent : 003C27; suivant : 003C29Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
Auteurs : RBID : Pascal:10-0370203Descripteurs français
- Pascal (Inist)
- Biodétecteur, Détecteur électrochimique, Chitosane, Polymère greffé, Nanoparticule, Particule métallique, Or, Couche ITO, Support, Immobilisation, Oligodésoxyribonucléotide, Fabrication, Copulation chimique, Morphologie, Caractéristique électrochimique, Voltammétrie cyclique, Limite détection, DNA complémentaire, Stabilité thermique, Stabilité stockage, Reproductibilité, Etude expérimentale.
- Wicri :
- concept : Or.
English descriptors
- KwdEn :
- Biosensor, Chemical coupling, Chitosan, Complementary DNA, Cyclic voltammetry, Detection limit, Electrochemical characteristic, Electrochemical detector, Experimental study, Gold, Graft polymers, ITO layers, Immobilization, Manufacturing, Metal particle, Morphology, Nanoparticle, Oligodeoxyribonucleotide, Reproducibility, Storage stability, Support, Thermal stability.
Abstract
A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16 nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03-325 fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572 μA/fM with a response time of 12 s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4 °C.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection</title><author><name>SHUNSHENG CAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Materials Science and Engineering, Jiangsu University</s1><s2>Zhenjiong 212 013</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Zhenjiong 212 013</wicri:noRegion></affiliation></author><author><name sortKey="Mishra, Rajeev" uniqKey="Mishra R">Rajeev Mishra</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Cancer Genetics, School of Medicine, Nihon University</s1><s2>Tokyo 173 8610</s2><s3>JPN</s3><sZ>2 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Tokyo 173 8610</wicri:noRegion></affiliation></author><author><name sortKey="Pilla, Srikanth" uniqKey="Pilla S">Srikanth Pilla</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Civil & Environmental Engineering, Stanford University</s1><s2>Stanford, CA 94305</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Stanford, CA 94305</wicri:noRegion></affiliation></author><author><name sortKey="Tripathi, Swapnil" uniqKey="Tripathi S">Swapnil Tripathi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Physics & Astronomy, University of Wisconsin-Barron County</s1><s2>Barron County, WI</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Barron County, WI</wicri:noRegion></affiliation></author><author><name sortKey="Pandey, Manoj K" uniqKey="Pandey M">Manoj K. Pandey</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho</s1><s2>Showa-ku, Nagoya-shi 466 8555</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Showa-ku, Nagoya-shi 466 8555</wicri:noRegion></affiliation></author><author><name sortKey="Shah, Gopit" uniqKey="Shah G">Gopit Shah</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Biological Sciences, University of Wisconsin-Milwaukee</s1><s2>Milwaukee, WI 53211</s2><s3>USA</s3><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Milwaukee, WI 53211</wicri:noRegion></affiliation></author><author><name sortKey="Mishra, Ajay K" uniqKey="Mishra A">Ajay K. Mishra</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Chemical Technology, University of Johannesburg, Doornfontein</s1><s2>Johannesburg 17011</s2><s3>ZAF</s3><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Afrique du Sud</country><wicri:noRegion>Johannesburg 17011</wicri:noRegion></affiliation></author><author><name sortKey="Prabaharan, Mani" uniqKey="Prabaharan M">Mani Prabaharan</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Chemistry, Faculty of Engineering and Technology, SRM University</s1><s2>Kattankulathur 603 203</s2><s3>IND</s3><sZ>8 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Kattankulathur 603 203</wicri:noRegion></affiliation></author><author><name sortKey="Mishra, Shivani B" uniqKey="Mishra S">Shivani B. Mishra</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Chemical Technology, University of Johannesburg, Doornfontein</s1><s2>Johannesburg 17011</s2><s3>ZAF</s3><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Afrique du Sud</country><wicri:noRegion>Johannesburg 17011</wicri:noRegion></affiliation></author><author><name>JIN XIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Materials Science and Engineering, Jiangsu University</s1><s2>Zhenjiong 212 013</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Zhenjiong 212 013</wicri:noRegion></affiliation></author><author><name sortKey="Pandey, R R" uniqKey="Pandey R">R. R. Pandey</name><affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Division of Engineering Materials, National Physical Laboratory</s1><s2>New Delhi 110 012</s2><s3>IND</s3><sZ>11 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>New Delhi 110 012</wicri:noRegion></affiliation></author><author><name>WEIWEI WU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Materials Science and Engineering, Jiangsu University</s1><s2>Zhenjiong 212 013</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Zhenjiong 212 013</wicri:noRegion></affiliation></author><author><name sortKey="Pandey, Avinash C" uniqKey="Pandey A">Avinash C. Pandey</name><affiliation wicri:level="1"><inist:fA14 i1="10"><s1>Nano Phosphor Application Centre, University of Allahabad</s1><s2>Allahabad 211 002</s2><s3>IND</s3><sZ>13 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Allahabad 211 002</wicri:noRegion></affiliation></author><author><name sortKey="Tiwari, Ashutosh" uniqKey="Tiwari A">Ashutosh Tiwari</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Materials Science and Engineering, Jiangsu University</s1><s2>Zhenjiong 212 013</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Zhenjiong 212 013</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="inist">10-0370203</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 10-0370203 INIST</idno><idno type="RBID">Pascal:10-0370203</idno><idno type="wicri:Area/Main/Corpus">004136</idno><idno type="wicri:Area/Main/Repository">003C28</idno></publicationStmt><seriesStmt><idno type="ISSN">0144-8617</idno><title level="j" type="abbreviated">Carbohydr. polym.</title><title level="j" type="main">Carbohydrate polymers</title></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensor</term><term>Chemical coupling</term><term>Chitosan</term><term>Complementary DNA</term><term>Cyclic voltammetry</term><term>Detection limit</term><term>Electrochemical characteristic</term><term>Electrochemical detector</term><term>Experimental study</term><term>Gold</term><term>Graft polymers</term><term>ITO layers</term><term>Immobilization</term><term>Manufacturing</term><term>Metal particle</term><term>Morphology</term><term>Nanoparticle</term><term>Oligodeoxyribonucleotide</term><term>Reproducibility</term><term>Storage stability</term><term>Support</term><term>Thermal stability</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Biodétecteur</term><term>Détecteur électrochimique</term><term>Chitosane</term><term>Polymère greffé</term><term>Nanoparticule</term><term>Particule métallique</term><term>Or</term><term>Couche ITO</term><term>Support</term><term>Immobilisation</term><term>Oligodésoxyribonucléotide</term><term>Fabrication</term><term>Copulation chimique</term><term>Morphologie</term><term>Caractéristique électrochimique</term><term>Voltammétrie cyclique</term><term>Limite détection</term><term>DNA complémentaire</term><term>Stabilité thermique</term><term>Stabilité stockage</term><term>Reproductibilité</term><term>Etude expérimentale</term></keywords><keywords scheme="Wicri" type="concept" xml:lang="fr"><term>Or</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16 nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03-325 fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572 μA/fM with a response time of 12 s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4 °C.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0144-8617</s0></fA01><fA02 i1="01"><s0>CAPOD8</s0></fA02><fA03 i2="1"><s0>Carbohydr. polym.</s0></fA03><fA05><s2>82</s2></fA05><fA06><s2>1</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection</s1></fA08><fA11 i1="01" i2="1"><s1>SHUNSHENG CAO</s1></fA11><fA11 i1="02" i2="1"><s1>MISHRA (Rajeev)</s1></fA11><fA11 i1="03" i2="1"><s1>PILLA (Srikanth)</s1></fA11><fA11 i1="04" i2="1"><s1>TRIPATHI (Swapnil)</s1></fA11><fA11 i1="05" i2="1"><s1>PANDEY (Manoj K.)</s1></fA11><fA11 i1="06" i2="1"><s1>SHAH (Gopit)</s1></fA11><fA11 i1="07" i2="1"><s1>MISHRA (Ajay K.)</s1></fA11><fA11 i1="08" i2="1"><s1>PRABAHARAN (Mani)</s1></fA11><fA11 i1="09" i2="1"><s1>MISHRA (Shivani B.)</s1></fA11><fA11 i1="10" i2="1"><s1>JIN XIN</s1></fA11><fA11 i1="11" i2="1"><s1>PANDEY (R. R.)</s1></fA11><fA11 i1="12" i2="1"><s1>WEIWEI WU</s1></fA11><fA11 i1="13" i2="1"><s1>PANDEY (Avinash C.)</s1></fA11><fA11 i1="14" i2="1"><s1>TIWARI (Ashutosh)</s1></fA11><fA14 i1="01"><s1>School of Materials Science and Engineering, Jiangsu University</s1><s2>Zhenjiong 212 013</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ><sZ>14 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Cancer Genetics, School of Medicine, Nihon University</s1><s2>Tokyo 173 8610</s2><s3>JPN</s3><sZ>2 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Civil & Environmental Engineering, Stanford University</s1><s2>Stanford, CA 94305</s2><s3>USA</s3><sZ>3 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Physics & Astronomy, University of Wisconsin-Barron County</s1><s2>Barron County, WI</s2><s3>USA</s3><sZ>4 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho</s1><s2>Showa-ku, Nagoya-shi 466 8555</s2><s3>JPN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="06"><s1>Department of Biological Sciences, University of Wisconsin-Milwaukee</s1><s2>Milwaukee, WI 53211</s2><s3>USA</s3><sZ>6 aut.</sZ></fA14><fA14 i1="07"><s1>Department of Chemical Technology, University of Johannesburg, Doornfontein</s1><s2>Johannesburg 17011</s2><s3>ZAF</s3><sZ>7 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="08"><s1>Department of Chemistry, Faculty of Engineering and Technology, SRM University</s1><s2>Kattankulathur 603 203</s2><s3>IND</s3><sZ>8 aut.</sZ></fA14><fA14 i1="09"><s1>Division of Engineering Materials, National Physical Laboratory</s1><s2>New Delhi 110 012</s2><s3>IND</s3><sZ>11 aut.</sZ></fA14><fA14 i1="10"><s1>Nano Phosphor Application Centre, University of Allahabad</s1><s2>Allahabad 211 002</s2><s3>IND</s3><sZ>13 aut.</sZ></fA14><fA20><s1>189-194</s1></fA20><fA21><s1>2010</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>19272</s2><s5>354000191707460290</s5></fA43><fA44><s0>0000</s0><s1>© 2010 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1/2 p.</s0></fA45><fA47 i1="01" i2="1"><s0>10-0370203</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Carbohydrate polymers</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16 nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03-325 fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572 μA/fM with a response time of 12 s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4 °C.</s0></fC01><fC02 i1="01" i2="X"><s0>001D10A08</s0></fC02><fC02 i1="02" i2="X"><s0>002A31C09B</s0></fC02><fC02 i1="03" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Biodétecteur</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Biosensor</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Biodetector</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Détecteur électrochimique</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Electrochemical detector</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Detector electroquímico</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Chitosane</s0><s5>04</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Chitosan</s0><s5>04</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Quitosano</s0><s5>04</s5></fC03><fC03 i1="04" i2="3" l="FRE"><s0>Polymère greffé</s0><s5>05</s5></fC03><fC03 i1="04" i2="3" l="ENG"><s0>Graft 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l="ENG"><s0>Support</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Soporte</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Immobilisation</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Immobilization</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Inmovilización</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Oligodésoxyribonucléotide</s0><s5>12</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Oligodeoxyribonucleotide</s0><s5>12</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Oligodesoxirribonucleótido</s0><s5>12</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Fabrication</s0><s5>14</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Manufacturing</s0><s5>14</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Fabricación</s0><s5>14</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>Copulation chimique</s0><s5>15</s5></fC03><fC03 i1="13" i2="X" l="ENG"><s0>Chemical coupling</s0><s5>15</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Copulación química</s0><s5>15</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Morphologie</s0><s5>16</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Morphology</s0><s5>16</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Morfología</s0><s5>16</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Caractéristique électrochimique</s0><s5>17</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Electrochemical characteristic</s0><s5>17</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Característica electroquímica</s0><s5>17</s5></fC03><fC03 i1="16" i2="X" l="FRE"><s0>Voltammétrie cyclique</s0><s5>18</s5></fC03><fC03 i1="16" i2="X" l="ENG"><s0>Cyclic voltammetry</s0><s5>18</s5></fC03><fC03 i1="16" i2="X" l="SPA"><s0>Voltametría cíclica</s0><s5>18</s5></fC03><fC03 i1="17" i2="X" l="FRE"><s0>Limite détection</s0><s5>19</s5></fC03><fC03 i1="17" i2="X" l="ENG"><s0>Detection limit</s0><s5>19</s5></fC03><fC03 i1="17" i2="X" l="SPA"><s0>Límite detección</s0><s5>19</s5></fC03><fC03 i1="18" i2="X" l="FRE"><s0>DNA complémentaire</s0><s1>ANA</s1><s5>20</s5></fC03><fC03 i1="18" i2="X" l="ENG"><s0>Complementary DNA</s0><s1>ANA</s1><s5>20</s5></fC03><fC03 i1="18" i2="X" l="SPA"><s0>DNA complementario</s0><s1>ANA</s1><s5>20</s5></fC03><fC03 i1="19" i2="X" l="FRE"><s0>Stabilité thermique</s0><s5>21</s5></fC03><fC03 i1="19" i2="X" l="ENG"><s0>Thermal stability</s0><s5>21</s5></fC03><fC03 i1="19" i2="X" l="SPA"><s0>Estabilidad térmica</s0><s5>21</s5></fC03><fC03 i1="20" i2="X" l="FRE"><s0>Stabilité stockage</s0><s5>22</s5></fC03><fC03 i1="20" i2="X" l="ENG"><s0>Storage stability</s0><s5>22</s5></fC03><fC03 i1="20" i2="X" l="SPA"><s0>Estabilidad al almacenamiento</s0><s5>22</s5></fC03><fC03 i1="21" i2="X" l="FRE"><s0>Reproductibilité</s0><s5>23</s5></fC03><fC03 i1="21" i2="X" l="ENG"><s0>Reproducibility</s0><s5>23</s5></fC03><fC03 i1="21" i2="X" l="SPA"><s0>Reproductividad</s0><s5>23</s5></fC03><fC03 i1="22" i2="X" l="FRE"><s0>Etude expérimentale</s0><s5>24</s5></fC03><fC03 i1="22" i2="X" l="ENG"><s0>Experimental study</s0><s5>24</s5></fC03><fC03 i1="22" i2="X" l="SPA"><s0>Estudio experimental</s0><s5>24</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Oside polymère</s0><s2>NK</s2><s5>03</s5></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Oside polymer</s0><s2>NK</s2><s5>03</s5></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Osido polímero</s0><s2>NK</s2><s5>03</s5></fC07><fN21><s1>235</s1></fN21><fN44 i1="01"><s1>PSI</s1></fN44><fN82><s1>PSI</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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